Chromatographie Liquide
Introduction to Modern Liquid Chromatography
by The latest edition of the authoritative reference to HPLCSnyder and
Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition,
with John Dolan as added coauthor, addresses important improvements in
columns and equipment, as well as major advances in our understanding
of HPLC separation, our ability to solve problems that were troublesome
in the past, and the application of HPLC for new kinds of samples.
This carefully considered Third Edition maintains
the strengths of the previous edition while significantly modifying its
organization in light of recent research and experience. The text
begins by introducing the reader to HPLC, its use in relation to other
modern separation techniques, and its history, then leads into such
specific topics as:
Equipment and detection. The column—the "heart" of the HPLC system.
Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques. Computer simulation, qualitative and quantitative analysis, and method validation and quality control. The separation of large molecules, including both biological and synthetic polymers
Chiral separations, preparative separations, and sample preparation. Systematic development of HPLC separations—new to this edition. Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms
Troubleshooting LC Systems: A Comprehensive Approach to Troubleshooting LC Equipment and Separations
by Over the last 15 years, high-performance liquid chromatogra phy (LC) has made the transition from an instrument used only by experts in research labs to a tool used for routine applications by relatively unskilled workers.With this transition have come in instrumentation and column technology. In major advances the past, the operator had to be a jack-of-all-trades, with a screw driver, soldering iron, and various wrenches as constant compan ions in the LC lab.Today, many instruments contain micropro cessors as powerful as those of mainframe computers of earlier days. With this technology has come a variety of self-diagnostic tools that allow the LC system to locate many of its own prob lems.
Traditionally, well-honed LC troubleshooting skills have been a result of years of work at the bench. Today the LC system itself often can do a better job of troubleshooting than the operator can. Yet many of the problems of the past are still the major problems of today: air bubbles, check valves, detector lamps, and, of course, problems with the separation.
An added pressure on the operator of today's LC system is that of productivity-the lab often cannot afford unnecessary downtime. This means that the operator has to be a troubleshooting expert, or has to have that expertise at his or her fingertips. The present book was written to provide this expertise in an easy-to-use format for users at all levels of experience.
Practical HPLC Method Development
by This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC.The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method.
This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.